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Part:BBa_K2371014:Design

Designed by: Wang Xiao   Group: iGEM17_BGIC-Union   (2017-10-24)


C-t7-dCas9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This biobrick is expected to generate the protein C-T7-dCas9 synthesized by part of split T7 polymerase and dCas9. The sequence of CT7 and dCas9 are linked via a linker. In consideration of the protein’s activity influenced by its function, the His-Tag must be linked to the C terminus of dCas9. Subparts: C-T7; linker; dCas9


Source

T7 polymerase from bacteria BL21(DE3); dCas9 from plasmid PX335

References